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Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
. Ed(S): Meier, Thomas; Fahrenholz, Falk
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Description for Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Paperback. Editor(s): Meier, Thomas; Fahrenholz, Falk. Series: Biomethods. Num Pages: 254 pages, biography. BIC Classification: MB; PSB; WM. Category: (P) Professional & Vocational. Dimension: 244 x 170 x 13. Weight in Grams: 454.
2.1 Introduction ...32 2.2 Technical Procedures ...32 Chemical Synthesis ...35 2.3 Results and Discussion...38 ...Chemical Synthesis ...38 Binding to anti-CCK antisera and CCK-B receptors ...40 Binding to streptavidin agarose and photoelution ...40 Affinity chromatography ...41 2.4 Troubleshooting...43 References ...44 ...Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ...45 Summary ...45 3.1 Introduction ...46 ...3.2 Technical Procedures ...48 Preparation of a Biotinylated Ligand ...48 Preparation of Membrane Fraction ...50 Solubilization of the PACAP Receptor ...51 Partial Purification of the PACAP Receptor ...52 Affinity Purification of the PACAP Receptor ...54 Final Purification of the PACAP Receptor ...56 ... Read more
2.1 Introduction ...32 2.2 Technical Procedures ...32 Chemical Synthesis ...35 2.3 Results and Discussion...38 ...Chemical Synthesis ...38 Binding to anti-CCK antisera and CCK-B receptors ...40 Binding to streptavidin agarose and photoelution ...40 Affinity chromatography ...41 2.4 Troubleshooting...43 References ...44 ...Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ...45 Summary ...45 3.1 Introduction ...46 ...3.2 Technical Procedures ...48 Preparation of a Biotinylated Ligand ...48 Preparation of Membrane Fraction ...50 Solubilization of the PACAP Receptor ...51 Partial Purification of the PACAP Receptor ...52 Affinity Purification of the PACAP Receptor ...54 Final Purification of the PACAP Receptor ...56 ... Read more
Product Details
Format
Paperback
Publication date
2012
Publisher
Springer Basel Switzerland
Number of pages
254
Condition
New
Series
Biomethods
Number of Pages
240
Place of Publication
Basel, Switzerland
ISBN
9783034873512
SKU
V9783034873512
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
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