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Signal Transduction - Single Cell Techniques
. Ed(S): Duijn, Bert, Van; Wiltink, Anneke
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Description for Signal Transduction - Single Cell Techniques
Paperback. Editor(s): Duijn, Bert, van; Wiltink, Anneke. Series: Springer Lab Manuals. Num Pages: 468 pages, biography. BIC Classification: MMG; MQW; PSB; PSF. Category: (G) General (US: Trade). Dimension: 246 x 189 x 26. Weight in Grams: 969.
A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH. ... Read more
A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH. ... Read more
Product Details
Format
Paperback
Publication date
2012
Publisher
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Germany
Number of pages
468
Condition
New
Series
Springer Lab Manuals
Number of Pages
468
Place of Publication
Berlin, Germany
ISBN
9783642489761
SKU
V9783642489761
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
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