From the reviews of the second edition: "This book is intended as a laboratory manual for researchers using Agrobacterium tumefaciens for plant transformation. It covers the basics of culturing and transforming Agrobacterium … . You don’t expect a lab manual to be ideal … reading, but I found this to be surprisingly readable. … The ... Read morebook would be especially useful for someone just starting plant transformation work or changing to new plant species and … it would also be useful reading for Masters students." (Heather Macdonald, Microbiology Today, November, 2006) "Methods in Molecular Biology opens … a field concerning Agrobacterium-mediated transformation of plants. … Each chapter is written by the leader in the field … and offers a detailed manual of the transformation protocol. … All users of this book will certainly appreciate the notes sections that bring additional information on potential difficulties in the protocols and alternative materials or methods. The book is the fountainhead of recent practical knowledge concerning Agrobacterium-mediated transformation and … it will become the core manual … ." (J. BRlZA, Biologia Plantarum, Vol. 51 (2), 2007) "The book’s chapters are organized in a highly detailed fashion, providing readers not only with step-by-step protocols … but also with a comprehensive list of equipment and materials. … With such a rich source of information, organized to be easy to read and very simple to implement, the book should be considered a must for every plant biology laboratory. … It will also be an excellent tool for training students, doctorates, technicians, and other laboratory personnel in the art of plant genetic transformation." (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, March, 2007) Show Less