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Differential-Display Reverse Transcription-Pcr (Ddrt-Pcr)
Colonna-Romano, Sergio; Leone, Antonella; Maresca, Bruno
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Description for Differential-Display Reverse Transcription-Pcr (Ddrt-Pcr)
Paperback. This manual describes the technique of differential-display reverse transcription-PCR. It contains step-by-step instructions, practical hints and experimental strategies for every protocol. Series: Springer Lab Manuals. Num Pages: 124 pages, 74 black & white illustrations, biography. BIC Classification: PSB; PSF. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 234 x 156 x 7. Weight in Grams: 202.
Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present ... Read more
Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present ... Read more
Product Details
Format
Paperback
Publication date
1998
Publisher
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Germany
Number of pages
124
Condition
New
Series
Springer Lab Manuals
Number of Pages
124
Place of Publication
Berlin, Germany
ISBN
9783540632979
SKU
V9783540632979
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
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