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Directed Enzyme Evolution: Screening and Selection Methods (Methods in Molecular Biology)
Frances H. Arnold (Ed.)
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Description for Directed Enzyme Evolution: Screening and Selection Methods (Methods in Molecular Biology)
Paperback. Editor(s): Arnold, Frances H.; Georgiou, George. Series: Methods in Molecular Biology. Num Pages: 383 pages, biography. BIC Classification: PSF. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 20. Weight in Grams: 534.
Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal ... Read more
Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal ... Read more
Product Details
Publisher
Humana Press
Format
Paperback
Publication date
2010
Series
Methods in Molecular Biology
Condition
New
Weight
534g
Number of Pages
383
Place of Publication
Totowa, NJ, United States
ISBN
9781617374722
SKU
V9781617374722
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
Reviews for Directed Enzyme Evolution: Screening and Selection Methods (Methods in Molecular Biology)
...covers a considerable number of protocols for a broad range of enzymes...very useful... - ChemBioChem