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High Performance Liquid Chromatography in Enzymatic Analysis

Edward F. Rossomando

€ 411.92

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Description for High Performance Liquid Chromatography in Enzymatic Analysis Hardcover. High performance liquid chromatography (HPLC) is a widely-used laboratory technique. In the second edition of this book, the author describes refinements of HPLC methods and explains and illustrates a range of laboratory applications. Series: Methods of Biochemical Analysis. Num Pages: 480 pages, Illustrations. BIC Classification: PNFC; PSBZ; PSD; PSF. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly. Dimension: 235 x 162 x 34. Weight in Grams: 852.
The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis.

High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their ... Read moreuse in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include:
* Application of HPLC to the assay of enzymatic activities
* Concepts and principles of HPLC, including the latest technological advances
* Concepts and principles of capillary electrophoresis (CE)
* Strategy for design of an HPLC/CE system for assay of enzyme activity
* Preparation of enzymatic activities from tissues and single cells
* Analysis of enzymatic activities in body fluids, including chromatobiosis
* HPLC for the identification of new enzymatic activities
* Fundamentals of the polymerase chain reaction
* HPLC in forensics
* Survey of enzymatic activities assayed by the HPLC method, including many new categories
* Multienzyme systems, including many new examples
* HPLC in the analysis of contaminated food

"It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition

Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field. Show Less

Product Details

Format

Hardback

Publication date

1998

Publisher

John Wiley and Sons Ltd United States

Number of pages

480

Condition

New

Series

Methods of Biochemical Analysis

Number of Pages

476

Place of Publication

New York, United States

ISBN

9780471103400

SKU

V9780471103400

Shipping Time

Usually ships in 7 to 11 working days

Ref

99-50

About Edward F. Rossomando

EDWARD F. ROSSOMANDO, DDS, PhD, is a professor in the Department of BioStructure and Function at the University of Connecticut Health Center in Farmington and the founder and Director of the Waterborne Disease Center, a research facility for the environmental sciences. Professor Rossomando is coeditor of the book Morphogenesis and serves as a member of the editorial board of the ... Read more

Reviews for High Performance Liquid Chromatography in Enzymatic Analysis

"This book discusses how to design a successful HPLC-based assay, and surveys many recent HPLC-based assays that have appeared in the primary literature."
The Quarterly Review of Biology, June 1999

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High Performance Liquid Chromatography in Enzymatic Analysis

Edward F. Rossomando

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