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Directed Enzyme Evolution
. Ed(S): Georgiou, George; Arnold, Frances H.
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Description for Directed Enzyme Evolution
Hardback. Editor(s): Georgiou, George; Arnold, Frances H. Series: Methods in Molecular Biology. Num Pages: 383 pages, biography. BIC Classification: PSAK1; PSBZ. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 229 x 152 x 31. Weight in Grams: 839.
Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal ... Read more
Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal ... Read more
Product Details
Format
Hardback
Publication date
2003
Publisher
Humana Press Inc. United States
Number of pages
383
Condition
New
Series
Methods in Molecular Biology
Number of Pages
383
Place of Publication
Totowa, NJ, United States
ISBN
9781588292865
SKU
V9781588292865
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
Reviews for Directed Enzyme Evolution
"...covers a considerable number of protocols for a broad range of enzymes...very useful..." - ChemBioChem