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Yogambigai Velmurugu - Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition - 9783319451282 - V9783319451282
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Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition

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Description for Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition Hardback. Series: Springer Theses. Num Pages: 199 pages, 7 black & white illustrations, 105 colour illustrations, 76 colour tables, biography. BIC Classification: PNFS; PSBC. Category: (P) Professional & Vocational. Dimension: 235 x 155 x 14. Weight in Grams: 502.

Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins.

Many cellular processes involve special proteins that bind to specific DNA sites with high affinity.  How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DNA at specific sites and indicate that DNA deformability is a key factor in site-specific ... Read more

The experiments presented in this thesis uncover, for the first time, rapid (~100-500 microseconds) DNA unwinding/bending attributed to nonspecific interrogation, prior to slower (~5-50 milliseconds) DNA kinking/bending/nucleotide-flipping during recognition. These results help illuminate how a searching protein interrogates DNA deformability and eventually “stumbles” upon its target site. Submillisecond interrogation may promote preferential stalling of the rapidly scanning protein at cognate sites, thus enabling site-recognition.  Such multi-step search-interrogation-recognition processes through dynamic conformational changes may well be common to the recognition mechanisms for diverse DNA-binding proteins. 

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Product Details

Format
Hardback
Publication date
2016
Publisher
Springer International Publishing AG Switzerland
Number of pages
199
Condition
New
Series
Springer Theses
Number of Pages
199
Place of Publication
Cham, Switzerland
ISBN
9783319451282
SKU
V9783319451282
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15

About Yogambigai Velmurugu
Yogambigai Velmurugu was awarded the PhD degree by the University of Illinois, Chicago, in 2015. 

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