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E. Coli Plasmid Vectors
. Ed(S): Casali, Nicola; Preston, Andrew
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Description for E. Coli Plasmid Vectors
Hardback. Editor(s): Casali, Nicola; Preston, Andrew. Series: Methods in Molecular Biology. Num Pages: 316 pages, biography. BIC Classification: PSAK. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 159 x 243 x 24. Weight in Grams: 628.
A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
Product Details
Format
Hardback
Publication date
2003
Publisher
Humana Press Inc. United States
Number of pages
316
Condition
New
Series
Methods in Molecular Biology
Number of Pages
316
Place of Publication
Totowa, NJ, United States
ISBN
9781588291516
SKU
V9781588291516
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
Reviews for E. Coli Plasmid Vectors
...a scientific review and a methodological "cook-book" of high quality. It is truly a highly practical handbook, useful for all scientists working with gene cloning and manipulation." - Folia Microbiologica "...a very useful addition to laboratories investigating plasmids per se, and for those employing plasmids as vectors for gene cloning." - Microbiology Today