Heterologous Gene Expression in E.Coli
Nicola A. . Ed(S): Burgess-Brown
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Description for Heterologous Gene Expression in E.Coli
Hardcover. Editor(s): Burgess-Brown, Nicola A. Series: Methods in Molecular Biology. Num Pages: 429 pages, 37 black & white illustrations, 55 colour illustrations, 53 colour tables, biography. BIC Classification: PSG. Category: (P) Professional & Vocational. Dimension: 254 x 178. .
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting ... Read more
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting ... Read more
Product Details
Format
Hardback
Publication date
2017
Publisher
Humana Press Inc. United States
Number of pages
429
Condition
New
Series
Methods in Molecular Biology
Number of Pages
429
Place of Publication
Totowa, NJ, United States
ISBN
9781493968855
SKU
V9781493968855
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
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