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Cytochrome P450 Protocols
Phillips, Ian R.; Shephard, Elizabeth A.
€ 186.55
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Description for Cytochrome P450 Protocols
Hardback. Presenting the techniques that use the methodologies for the investigation of P450s, this title includes protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases, expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Series: Methods in Molecular Biology. Num Pages: 364 pages, 47 black & white illustrations, biography. BIC Classification: MMGT; PSB; PSD. Category: (P) Professional & Vocational. Dimension: 240 x 162 x 28. Weight in Grams: 714.
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, ... Read more
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, ... Read more
Product Details
Format
Hardback
Publication date
2005
Publisher
Humana Press Inc. United States
Number of pages
364
Condition
New
Series
Methods in Molecular Biology
Number of Pages
364
Place of Publication
Totowa, NJ, United States
ISBN
9781588294418
SKU
V9781588294418
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
Reviews for Cytochrome P450 Protocols
From Reviews of the First Edition... "...a remarkable book, highly useful for all our scientific readers..." -Cellular and Molecular Biology "...will be welcomed by laboratory scientists of various profiles who need authoritative protocols for their studies." -Modern Pathology From the reviews of the second edition: "In this second edition, editors Phillips ... Read more