Nuclear Reprogramming
Steve . Ed(S): Pells
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Description for Nuclear Reprogramming
hardcover. A collection of reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Editor(s): Pells, Steve. Series: Methods in Molecular Biology. Num Pages: 334 pages, biography. BIC Classification: PSD; PSF. Category: (P) Professional & Vocational. Dimension: 235 x 155 x 25. Weight in Grams: 747.
A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, ... Read more
A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, ... Read more
Product Details
Format
Hardback
Publication date
2005
Publisher
Humana Press Inc. United States
Number of pages
334
Condition
New
Series
Methods in Molecular Biology
Number of Pages
334
Place of Publication
Totowa, NJ, United States
ISBN
9781588293794
SKU
V9781588293794
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
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