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Methods of Protein Microcharacterization
John E. Shively
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Description for Methods of Protein Microcharacterization
Paperback. Series: Biological Methods. Num Pages: 456 pages, biography. BIC Classification: PSB. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 25. Weight in Grams: 697.
Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti- tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of ... Read more
Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti- tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of ... Read more
Product Details
Format
Paperback
Publication date
2013
Publisher
Humana Press Inc. United States
Number of pages
456
Condition
New
Series
Biological Methods
Number of Pages
456
Place of Publication
Totowa, NJ, United States
ISBN
9781489941329
SKU
V9781489941329
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
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