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PRINS and in Situ PCR Protocols
Franck . Ed(S): Pellestor
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Description for PRINS and in Situ PCR Protocols
Hardback. Describes in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Editor(s): Pellestor, Franck. Series: Methods in Molecular Biology. Num Pages: 244 pages, biography. BIC Classification: PSAK; PSD. Category: (P) Professional & Vocational. Dimension: 235 x 155 x 23. Weight in Grams: 563.
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing ... Read more
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing ... Read more
Product Details
Format
Hardback
Publication date
2006
Publisher
Humana Press Inc. United States
Number of pages
244
Condition
New
Series
Methods in Molecular Biology
Number of Pages
244
Place of Publication
Totowa, NJ, United States
ISBN
9781588295491
SKU
V9781588295491
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
Reviews for PRINS and in Situ PCR Protocols
From Reviews of the First Edition... "...essential for any laboratory contemplating exploiting this powerful technique." -Microbiology Today "...[a] splendid little book which covers the field quite well,,," -FEBS Letters "...accessible and very practical...recommend[ed]...to any laboratory with an operational PCR machine...a valuable addition to the methods literature." -Quarterly Review of Biology ... Read more