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Quantitation of MRNA by Polymerase Chain Reaction
Kohler, Thomas; Lassner, Dirk; Rost, A..K.; Thamm, B.; Pustowoit, B.; Remke, H.
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Description for Quantitation of MRNA by Polymerase Chain Reaction
Paperback. Series: Springer Lab Manuals. Num Pages: 166 pages, 17 black & white illustrations, 10 black & white tables, biography. BIC Classification: MFN; PNF; PSB. Category: (P) Professional & Vocational. Dimension: 234 x 156 x 10. Weight in Grams: 305.
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or ... Read more
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or ... Read more
Product Details
Format
Paperback
Publication date
2011
Publisher
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Germany
Number of pages
166
Condition
New
Series
Springer Lab Manuals
Number of Pages
166
Place of Publication
Berlin, Germany
ISBN
9783642797149
SKU
V9783642797149
Shipping Time
Usually ships in 15 to 20 working days
Ref
99-15
Reviews for Quantitation of MRNA by Polymerase Chain Reaction
"...this technical book will be very useful for all customers of PCR. And they are numerous!... Summarizing: an excellent booklet, which we recommend highly and absolutely necessary to all practicians of PCR products and their applications." Cellular and Molecular Biology